- Species: Saccharomyces cerevisiae
- Host Species/Expression System: E.coli
- Molecular Weight: 27.8 kDa
- Purity: ≥ 95 % by SDS-PAGE analysis
- Format: Liquid
- Tag: 6x His tag at N-term
- Biological Activity: Cleavage efficiency depends on the distance between the SUMO tag and the protein. Generally, a 1:200 ratio with one-hour incubation at room temperature achieves >99% SUMO tag cleavage.
- Formulation: 50% Glycerol, 5 mM DTT, 150 mM NaCl, 20 mM Tris pH7.8
- Storage Condition: Store at -20℃ for 2 years, or at 2-8℃ for up to 1 week. Avoid repeated freeze/thaw cycles.
- Synonyms: ULP1 peptidase, Ubiquitin-like-specific protease 1
- Unit Definition: One unit of SUMO Protease (ULP1) cleaves > 90% of 2 μg control substrate at 30°C for 1 h.
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