Gene Synthesis
基因合成服務
Gene synthesis is a revolutionary technology. Unlike DNA replication in cells and amplification via PCR, gene synthesis does not require DNA templates to synthesize target gene sequences.
基因合成是一個具有革命性的技術，與在細胞中發生的DNA複製及經由PCR放大不同，基因合成不需要DNA模板就能合成目標基因序列，幾乎任何序列都可以合成。
The Service / 服務內容
Streamline your gene insertion process with our complete gene synthesis service. No more tedious PCR and cloning from cDNA libraries or existing templates. Just provide us with your desired gene sequence, protein sequence, or NCBI information, and we’ll design optimized sequences tailored to your needs.
想要在特定質體中嵌入想表現的基因，不再需要從cDNA library 或者既有的DNA模板中反覆 PCR 和clone了！全基因合成從無到有，直接讓您獲得一個嵌入在完整質體中的DNA序列！
您只需要簡單提供我們欲合成的基因序列、欲表達的蛋白質序列或是NCBI上的資訊，我們將會依照您的需求幫您設計相對應的優化序列，並於最短的交付日期內提供序列100%正確無誤的產品。
Why Apical Lab / 服務特色
Our gene synthesis technology involves synthesizing target sequences as small oligo fragments before assembling them into larger fragments. This enables rapid and 100% accurate generation of the desired DNA sequence.
全基因合成技術是先將目標序列合成小片段 Oligo 後，再進行大片段的拼接。所以能快速而且100%序列正確的生成目標 DNA序列。
We can synthesize conventional sequences ranging from a few tens of base pairs to 1 megabase pair, as well as special structural sequences (Hairpin structure, GC-rich, Repeated sequence).
我們可以合成短至數十 bp、長至 1 Mbp的常規序列，或者特殊結構序列 (Hairpin structure、GC-rich、Repeated sequence) 。
Our technical team offers complimentary sequence optimization for enhancing the expression levels of your target protein across different host cells.
我們的技術團隊將免費為您提供的序列進行優化，針對不同的宿主細胞來提高您的目標蛋白表現量。
We offer a diverse range of almost a hundred vectors for you to choose from. No more need for manual subcloning!
我們有近百種載體可以讓您選擇。您不再需要自行 Sub-cloning！
Process / 服務流程
You can simply provide basic gene information or provide a detailed sequence, and indicate the desired vector to be used.
您可以簡單提供基因訊息，或者詳細地列出序列，並確認是否進行序列優化，以及欲使用的載體。
We will contact you to confirm service requirements and project specifications, and provide detailed pricing and delivery information.
我們會與您聯繫，確認服務需求與專案規格，並提供詳細報價與交期資訊。
We deliver plasmids containing synthesized genes with a yield of >5ug, accompanied by sequencing reports and a product documentation report.
我們提供 >5ug 含有合成基因的質體，並提供電子檔定序報告，以及產品報告書。
More than Gene Synthesis
不僅是合成基因
Apical Lab provides comprehensive development services that include plasmid design and sequence optimization during gene synthesis, ensuring a smoother process for protein development and antibody production.
Apical Lab 提供全面性的開發服務，於基因合成時，便為您進行質體設計與序列優化，讓您在後續蛋白質開發與抗體生產時，更加的順利！
Frequently Asked Questions
常見問題
Gene Synthesis Timeline?
基因合成需要多久時間？
Regular sequences within 1500bp can be completed within 20 working days.
1500bp 以內的普通序列，可以在 20 個工作日內可以完成。最急件可在 7 個工作日內完成。
What is Sequence Optimization?
序列優化是什麼？
Sequence optimization involves replacing the DNA sequence of a recombinant protein with commonly used codons in the host organism, as foreign codons may lead to insufficient protein expression. This optimization allows the recombinant DNA to express the protein more effectively in the host organism, thereby increasing production yield.
DNA可能因為包含外源宿主不常使用的密碼子，而導致重組蛋白質表現量不足。序列優化即是依照外源宿主常用的密碼子，更換重組DNA的序列，使其可以在外源宿主中表達重組蛋白，以提高產量。
Advantages of Gene Synthesis?
與傳統PCR克隆相比，基因合成有什麼優點？
Gene synthesis offers several advantages over traditional PCR cloning. Synthesized genes are mutation-free and do not require a DNA template, thereby avoiding restrictions related to gene sources and restriction enzyme sites. Additionally, optimized sequences can significantly enhance protein expression levels.
基因合成獲得的基因無突變、不需要DNA模板，因而不受基因來源限制及酶切位。且優化後的序列能大幅提高蛋白質表達量。
In addition to gene synthesis, do you provide protein expression services?
除了合成基因，是否還提供蛋白質表達服務？
By choosing Apical for gene synthesis, you can seamlessly proceed to our protein expression services or even advance to antibody development.
選擇在 Apical Lab 合成基因，可以以最優化的序列設計無縫接軌 Apical Lab 蛋白質表達服務，或進行一步完成抗體開發。
Do you have any case studies that I can refer to?
有任何案例分享嗎？
We regularly share case studies on Facebook and Instagram. Please refer to the following URLs:
我們不定期在 Facebook 與 Instagram 分享客戶案例，請參考以下連結：
Facebook: https://www.facebook.com/apicallab
Instagram: https://www.instagram.com/apicallab
