N-GlyFind™ GST Kit

N-GlyFind™ GST Kit 為源自小鼠 Fbs1 (Fbxo2) 的重組蛋白，並且嵌合GST標籤，提供比原產品 N-GlyFind™ Core-Specific Reagent Kit 十倍以上的靈敏度以檢測 ManGlcNAc2-Asn，一個位於醣蛋白中常見的 N-聚糖核心結構。應用範圍包含 ELISA、Western blot、Flow 及其他分析檢測中作為一級探針使用。本產品可選擇具 Biotin Conjugation 的版本，以利用 Streptavidin 系統進行訊號呈現。本產品具 8x His Tag，可利用 His Tag Antibody 辦識，或透過 FasTEV™（#AP9020）去除；FasTEV™ 是一種具有增強穩定性與催化活性的 TEV 蛋白酶。

Package: 1 mg

Conjugation: None / Biotin

Official Name: N-GlyFind™ GST Kit
Description: The reagent contains a fusion protein of glutathione S-transferase (GST) and the original N-GlyFind™ Core-Specific Reagent engineered from Mus musculus Fbs1 (Fbxo2). This highly purified affinity reagent is about 10X more sensitive than the original N-GlyFind™ Core-Specific Reagent, and is designed for robust and specific detection of ManGlcNAc2-Asn within the N-glycan core structure commonly found in glycoproteins. Applications include use as a primary probe in ELISA, Western blot, flow cytometry, and other assays.
Application: ELISA, WB, Flow
Tag: 8x His Tag
Conjugate: Biotin upon request
Content: Purified lyophilized powder
Storage Buffer: 50 mM EPPS, 200 mM NaCl, pH 7.5
Storage: -20 °C as powder. Once reconstituted, 4 °C for five days or -20 °C for up to six months.

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