N-GlyFind™ Core-Specific Reagent Kit

N-GlyFind™ Core-Specific Reagent Kit 為源自小鼠 Fbs1 (Fbxo2) 的重組蛋白,其可穩定且具專一性地檢測 ManGlcNAc2-Asn,一個位於醣蛋白中常見的 N-聚糖核心結構。應用範圍包含在酵素免疫分析法 (ELISA)、西方墨點法 (Western blot) 及其他分析檢測中作為一級探針使用。本產品可選擇具 Biotin Conjugation 的版本,以利用 Streptavidin 系統進行訊號呈現,或選擇 SureLight™ 488 Conjugation 以螢光偵測。本產品具 8x His Tag,可利用 His Tag Antibody 辦識,或透過 FasTEV™(#AP9020)去除;FasTEV™ 是一種具有增強穩定性與催化活性的 TEV 蛋白酶。

Package: 1 mg

Conjugation: None / Biotin / SureLight 488

SKU: AP9005-2 Category: Tags: ,
  • Official Name: N-GlyFind™ Core-Specific Reagent Kit
  • Description: The reagent contains a recombinant protein engineered from Mus musculus Fbs1 (Fbxo2), a component of the E3 ubiquitin ligase complex. This highly purified affinity reagent is designed for sensitive, robust, and specific detection of ManGlcNAc2-Asn within the N-glycan core structure commonly found in glycoproteins. Applications include use as a primary probe in ELISA, Western blot, and other assays. Each kit also includes 5X Tris Buffered Saline (BA0104) as a binding buffer to ensure maximum reagent specificity and ease of use.
  • Application: ELISA, WB
  • Tag: 8x His Tag
  • Conjugate: Selected from Biotin or SureLight 488
  • Content: Purified lyophilized powder
  • Storage Buffer: 50 mM EPPS, 200 mM NaCl, pH 7.5
  • Storage: -20 °C as powder. Once reconstituted, 4 °C for five days  or -20 °C for up to six months.

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