- Official Name: Pan Sialidase
- Specificity: Sialidase (Pan) catalyzes the hydrolysis of terminal α-linked N-acetylneuraminic acid (Neu5Ac) from oligosaccharides, complex carbohydrates, and glycoproteins. No detectable activities of proteases or other glycosidases are observed.
- Content: Purified lyophilized powder
- Tag: 8x His tag at N-term, which can be removed by digestion with FasTEV™
- Reconstitution: Dissolve the lyophilized product in 100 μL of molecular grade water to make a 50,000 units/ml solution in the buffer.
- Buffer: 50 mM EPPS, 100 mM NaCl, pH 7.5
- Storage: -20 °C as powder. Once reconstituted, 4 °C for five days or -20 °C for up to three months. Avoid freeze-thaw cycles.
- Unit Definition: One unit is defined as the amount of Sialidase (Pan) required to catalyze the release of 1 nanomole of p-nitrophenol (pNP) from 2-O-(p-nitrophenyl)-α-D-N-acetylneuraminic acid (pNP-Neu5Ac) in 1 min at 37°C in 100 μL 1X Reaction Buffer 4 (50 mM Tris-HCl, 100 mM NaCl, pH 7.5).
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