PNGase F-II
Pngase F Ii Data.png

PNGas F-II

PNGas F-II 能有效地從醣蛋白中切除幾乎所有 N-link 寡醣，而且毫不受 fucose 的干擾。PNGase F 屬於酰胺酶 (amidase) 的一種，能特異性地在 GlcNAc 與 asparagine 之間進行切割，並完整保留 N-link 寡醣的主要核心結構，使反應後的產物適合更進一步的分析。

Package: 100 U

SKU: AP9012 Category: Tags: ,
  • Official Name: PNGas F-II
  • Specificity: The PNGase F enzyme catalyzes the cleavage of asparagine-linked oligosaccharides, with or without core fucose, from glycoproteins and glycopeptides. No detectable activities of proteases or other glycosidases are observed.
  • Content: Purified lyophilized powder
  • Reconstitution: Dissolve the lyophilized product in 100 μL ultrapure water to make a 1,000 units/mL solution in the Buffer.
  • Buffer: 20 mM Tris-HCL, 200 mM NaCl, pH 7.5
  • Storage: -20 °C as powder. Once reconstituted, 4 °C for five days  or -20 °C for up to six months. Avoid freeze-thaw cycles.
  • Unit Definition: One unit is defined as the amount of PNGase F-II required to deglycosylate 1 nanomole (15 μg) of denatured RNase B or 0.1 nanomole (4.5 μg) of horse radish peroxidase (HRP) in 2 h at 37°C in 25 μL 1X Reaction Buffer (50 mM Bis-Tris, 100 mM NaCl, pH 7.0).

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