Anti-GFP VHH Antibody | JotBody

GFP / EGFP VHH Antibody

綠色螢光蛋白（GFP）是一種由水母 Aequorea victoria 衍生而來的蛋白質，含有238個氨基酸殘基（26.9kDa），當受到藍光（excitation: 395nm）激發時，會發出綠光（emission: 509nm）。GFP在細胞生物學研究中成為一種極其珍貴的工具，因為它內在的螢光可以在活體細胞中被視覺化。而 EGFP 包含兩個胺基酸置換，即 Phe-64 變為 Leu 和 Ser-65 變為 Thr（PMID: 8707053）。與野生型 GFP（wtGFP）相比，EGFP有一個單一、強烈以及紅移的激發光於 488nm。抗 GFP VHH antibody 是基於 VHH 抗體的重組單株抗體，專門針對 GFP / EGFP 蛋白進行辨識，並以其高度的組織穿透力、結構穩定性和重組可塑性而廣為使用。

Official Name: Anti-GFP VHH Antibody
Host: Alpaca
Clonality: Recombinant
Clone Name: GE10
Conjugation: Un-conjugated; with a 6xHis tag at its C-terminal
Application: ELISA, WB, IF, IP
Reactivity: Species independent
Formulation: 25 mM TAPS pH8.5, 500 mM NaCl, 5 mM EDTA, 0.1 % Proclin 300 Solution.
Storage: 4℃. Do not freeze.
Description: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr (previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488 nm.

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