FasTEV Protease

FasTEV™ Protease (His tag)

FasTEV™ Protease 是一經由基因工程技術優化的 TEV Protease，其中融入了多種精心設計的突變，旨在提升其結構穩定性及催化效率。該酵素具有高度的專一性，能夠針對 ENLYFQ↓(G/S) 序列中的 Glutamic acid 與 Glycine 或 Serine 之間的鍵結進行精確的切割。在蛋白質工程領域，FasTEV™ Protease 常被應用於與 TEV cleavage site 結合的策略中，以便於有效地去除蛋白質上的各種標籤，進而達到純化或功能性研究的目的。

Package: 1000 U

SKU: AP9020 Category: Tags: ,
  • Official Name: FasTEV™ Protease
  • Specificity: The enzyme specifically cleaves the peptide bond between a glutamine and a glycine or serine of the consensus sequence, ENLYFQ↓(G/S). No detectable activities of proteases or other glycosidases are observed.
  • Content: Purified lyophilized powder
  • Tag: 8x His tag at N-term, which can be removed by digestion with FasTEV™
  • Reconstitution: Dissolve the lyophilized product in 100 μL molecular grade water to make a 10,000 units/mL solution in the buffer.
  • Buffer: 50 mM Tris, 50 mM NaCl, 1 mM EDTA, pH 7.5
  • Storage: -20 °C as powder. Once reconstituted, 4 °C for two weeks  or -20 °C for up to three months. Avoid freeze-thaw cycles.
  • Unit Definition: One unit is defined as the amount of FasTEVTM required to cleave 90% of 0.1 nanomole (5.4 μg) of a fusion protein substrate in 1 h at 30°C in 20 μL 1X Reaction Buffer (50 mM Tris, 50 mM NaCl, 1 mM EDTA, pH 7.5).

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