- Official Name: Endo S
- Specificity: The enzyme catalyzes the cleavage of N-linked glycans from IgG heavy chain. No detectable activities of proteases or other glycosidases are observed.
- Content: Purified lyophilized powder
- Tag: 8x His tag at N-term, which can be removed by digestion with FasTEV™
- Reconstitution: Dissolve the lyophilized Endo S in 100 μL molecular grade water to make a 25,000 units/mL solution in the buffer.
- Buffer: 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5
- Storage: -20 °C as powder. Once reconstituted, 4 °C for seven days or -20 °C for up to three months. Avoid freeze-thaw cycles.
- Unit Definition: One unit is defined as the amount of Endo-S required to deglycosylate > 90% of 2.5 μg of human IgG in 1 h at 37°C in 25 µL 1X Reaction Buffer 6 (50 mM NaOAc, 5 mM CaCl2, pH 5.5).
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