alpha 2,3 Sialidase (His tag)

alpha 2,3 Sialidase 透過辦識 alpha 2,3 sialyl linkage 專一地從醣蛋白、醣脂或碳水化合物上切除 Neu5Ac。反應後的產物結構能完整保留，以進行更進一步的分析。

Package: 5000 U

Official Name: alpha 2,3 Sialidase
Specificity: The enzyme releases terminal α2,3-linked N-acetylneuraminic acid (Neu5Ac) from oligosaccharides, complex carbohydrates, and glycoproteins. No detectable activities of proteases or other glycosidases are observed.
Content: Purified lyophilized powder
Tag: 8x His tag at N-term, which can be removed by digestion with FasTEV^™
Reconstitution: Dissolve the lyophilized powder in 100 μL of ultrapure water to make a 50,000 units/ml solution in the buffer.
Buffer: 50 mM EPPS, 100 mM NaCl, pH 7.5
Storage: -20 °C as powder. Once reconstituted, 4 °C for five days or -20 °C for up to six months. Avoid freeze-thaw cycles.
Unit Definition: One unit is defined as the amount of α2,3 Sialidase required to catalyze the release of 1 nanomole of p-nitrophenol (pNP) from 2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid (pNP-Neu5Ac) in 1 min at 37°C in 100 μL 1X Reaction Buffer 4 (50 mM EPPS, 100 mM NaCl, pH 7.5).

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