- Official Name: beta 1,3 Galactosidase
- Specificity: beta 1,3 Galactosidase catalyzes the hydrolysis of terminal β1,3-linked galactose (Gal) from oligosaccharides and glycoprotein substrates. No detectable activities of proteases or other glycosidases are observed.
- Content: Purified lyophilized powder
- Tag: 8x His tag at N-term, which can be removed by digestion with FasTEV™
- Reconstitution: Dissolve the lyophilized product in 100 μL molecular grade water to make a 20,000 units/mL solution in the buffer.
- Buffer: 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5
- Storage: -20 °C as powder. Once reconstituted, 4 °C for seven days or -20 °C for up to three months. Avoid freeze-thaw cycles.
- Unit Definition: One unit is defined as the amount of β1,3 Galactosidase required to catalyze the release of 1 nmole p-nitrophenol (pNP) from p-nitrophenyl-β-D-galactopyranoside (pNP-Gal) in 10 min at 37°C in 100 μL 1X Reaction Buffer 1 (20 mM Tris, 50 mM NaCl, 1 mM EDTA pH 7.5).
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