- Official Name: Thermophilic Fucosidase
- Specificity: Thermophilic Fucosidase is active at a wide range of temperatures (37 to 97°C), with an optimum of 70°C or higher. At 37°C, it primarily releases α1,2-linked fucose. At 70°C, it preferentially cleaves Fucα1,2 > Fucα1,3 ≈ Fucα1,4 ≈ Fucα1,6 linkages. No detectable activities of proteases or other glycosidases are observed.
- Content: Purified lyophilized powder
- Tag: 8x His tag at N-term, which can be removed by digestion with FasTEV™
- Reconstitution: Dissolve the lyophilized product in 100 μL of molecular grade water to make a 2,000 units/mL solution in the Buffer.
- Buffer: 50 mM Tris, 100 mM NaCl, pH 7.5
- Storage: -20 °C as powder. Once reconstituted, 4 °C for five days or -20 °C for up to six months. Avoid freeze-thaw cycles.
- Unit Definition: One unit is defined as the amount of Thermophilic Fucosidase required to catalyze the release of 1 nmole p-nitrophenol (pNP) from p-nitrophenyl-α-L-fucopyranoside (pNP-Fuc) per min at 37°C in 100 μL 1X Reaction Buffer 4 (50 mM Tris, 100 mM NaCl, pH 7.5).
立即詢價