- Species: Human
- Host Species/Expression System: E.coli
- Molecular Weight: 63.5 kDa
- Purity: ≥ 95 % by SDS-PAGE analysis
- Format: Lyophilized
- Biological Activity: Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is typically 1-5 μg/ cm2. Fibronectin can also be added to the media to support cell spreading at a concentration of 0.5-50μg/ mL
- Endotoxin: < 1.0 EU/μg as determined by LAL method
- Formulation: 20 mM Tris-HCl, pH 8.0, 250 mM NaCl, with sucrose
- Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile ddH2O to a concentration of 0.1-10 mg/mL. Stock solutions should be apportioned into working aliquots and store at ≤ -20°C. Future dilutions should be made in appropriate buffered solution.
- Storage Condition: Store the lyophilized protein at -20℃ to -80℃ for lone term. After reconstitution, the protein solution is stable at -20℃ for 3 month, at 2-8℃ for up to 1 week. Avoid repeated freeze/thaw cycles
- Amino Acid Sequence: MPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSMAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTHHHHHH
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