O-GlcNAcase (OGA)

O-GlcNAcase (OGA) 酵素可高度專一的辦識醣蛋白上與 serine 或 threonine 胺基酸以 O-link 鏈結的 GlcNAc,並進行切除。這個反應在 Tau 蛋白的去磷酸化過程中十分重要,因此 OGA 酵素時常見於阿茲海默症的研究中。

Package: 1000 U

SKU: AP9013 Category: Tags: ,
  • Official Name: O-GlcNAcase
  • Specificity: The enzyme releases O-linked β-N-acetylglucosamine (GlcNAc) from serine and threonine residues of glycoproteins and glycopeptides. No detectable activities of proteases, exo-β-N-acetylglucosaminidase, or other glycosidases are observed.
  • Content: Purified lyophilized powder
  • Reconstitution: Dissolve the lyophilized product in 100 μL of ultrapure water to make a 10,000 units/mL in buffer.
  • Buffer: 50 mM Tris, 100 mM NaCl, pH 7.5
  • Storage: -20 °C as powder. Once reconstituted, 4 °C for five days  or -20 °C for up to three months. Avoid freeze-thaw cycles.
  • Unit Definition: One unit is defined as the amount of O-GlcNAcase required to catalyze the release of 1 nmole p-nitrophenol (pNP) from p-nitrophenyl-N-acetyl-β-D-glucosaminide (pNP-GlcNAc) per min at 37°C in 100 μL 1X Reaction Buffer (50 mM Tris, 100 mM NaCl, pH 7.5).