alpha 1,3 Mannosidase (His tag)

alpha 1,3 Mannosidase 可特異性地將 α1,3 mannose  從醣鏈上切除。這個機制在多種生物功能上都有其重要性,包含蛋白質修飾、免疫反應、細胞訊息傳遞,以及病原體感染等。

Package: 1000 U

SKU: AP9017 Category: Tags: ,
  • Official Name: alpha 1,3 Mannosidase
  • Specificity: alpha 1,3 Mannosidase catalyzes the hydrolysis of terminal α1,3-linked mannose (Man) residues from oligosaccharides and glycoprotein substrates. No detectable activities of proteases or other glycosidases are observed.
  • Content: The product is supplied as a 10,000 units/mL solution in the buffer.
  • Tag: 8x His tag at N-term, which can be removed by digestion with FasTEV
  • Buffer: 20 mM Tris-HCl, 100 mM NaCl, pH 7.5
  • Storage: 4 °C for seven days, or -80 °C for up to six months. Avoid freeze-thaw cycles.
  • Unit Definition: One unit is defined as the amount of α1,3 Mannosidase required to catalyze the release of 1 nmole 4-methylumbelliferone (4MU) from 4-methylumbelliferyl α-D-mannopyranoside (4MU-Man) in 30 min at 37°C in 50 μL 1X Reaction Buffer 2 (50 mM Bis-Tris, 100mM NaCl, pH 7.0), plus 0.2 mM ZnSO4 and 1 mg/mL BSA.